연(蓮)의 부위에 따른 구강위생균에 대한 항균작용

Abstract

To prevent of periodontal disease and tooth decay to explore the antimicrobial substances, the substances from methanol extract of Nulembo nucifera which grows naturally in Korea were distinguished by part. (Flower, Leaf, Seed, Pod) Antimicrobial effect against five microorganisms (S.mutans, S. sobrinus KCCM11897 S.sobrinus KCCM11898, F.nucleatum , A.actinomycetemcomitans) were mesured by paper disc method and the growth inhibition rate. Then Minimum inhibitory concentrarion(MIC) was used know the antimicrobial action of extract and then antimicrobial after looking at these temperatures whether how stable the following results were obtained. The results were obtained as follow : 1) Lotus flower showed the highest yield : MeOH 21% and chloroform fraction is 4.2%, ethylacetate fraction is 8.2%, water fraction is 19%. 2) Lotus flower and leaf the methanol concentration to 50% were the most anti-microbial. However, seed and pod with a methanol concentration of 70% was the most effective results. Also, In the extraction of 6 hours and 12 hours were superior antimicrobial activity. And then antimicrobial activity was reduced as time goes on. Accordingly, antimicrobial activity takes place all the extraction within 12 hour. 3) When examined about antimicrobial activity of methanol extract(MEex), chloroform fraction(Chfr), ethylacetate fraction (EAfr), and water fraction(WAfr) using paper disc method, EAfr showed an anti oral microbial effect against all test microorganisms. 4) Lotus flower and seed extract showed strong inhibition against S.sobrinus KCCM 11897, also S.sobrinus KCCM 11898 was inhibitied by seed extract. F.nucleatum was strongly inhibited by leaf extract and this was the most effective against A. actinomycetemcomitans. 5) Inhibition rate of Lotus leaf was 50% against S.mutans and S.sobrinus. In both F.nucleatum and A.actinomycetemcomitans lotus flower 40% inhibition was observed. 6) MICs of microorganisms from extract by broth microdilution method lead as follows. Flower extracts against S.sobrinus had concentration of 625 µg/ml, and leaf extracts also had concentrations of 625 µg/ml as a test showed the lowest MICs value. 7) After heat treatment of extracts at 40, 80, 100 and 120℃ for an hour growth rate of oral microorganism was checked. All extracts with heat at 120 ℃ had antimicrobial activity, which means that is a very stable substances. 8) In result of growth of S.mutans and S.sobinus in the medium adding extracts treated various pH conditions (2, 5, 7, 9, 11) there was no difference of antimicrobial activity. So its stability on pH was high quality. While F.nucleatum and A. actinomycetemcomitans was stable against acid it had a trend that the more alkai side was the lower activity. In the past, lotus with a strong antibiotic and detoxifying substances have been used in a variety of diseases. The effect of lotus to prevent oral disease but was insufficient to prove scientifically. By oral bacteria that cause oral disease in many ways a look at the antimicrobial activity, the effects appears differently for each parts by lotus. This means that lotus different parts showed that the optional available. Based on the results of this study, after finding substance to inhibit microbacteria if applied to another oral bacteria, lotus will be available in a variety as a oral hygiene.