고상발효 기질에서 CoQ10과 천연 스타틴(natural statin) 생산에 대한 홍국균(Monascus sp.)과 황국균(Aspergillus sp.)의 영향

Abstract

Mevinolin (natural statins), a fungal metabolite, is a potent inhibitor of HMG-CoA reductase, the rate-controlling enzyme in cholesterol biosynthesis. Ubiquinone (Coenzyme Q10, CoQ10) is a lipid soluble, endogenous hydroxy benzoquinone compound involved in electron transport in the mitochondrial oxidative phosphorylation, and it protects membrane phospholipids against peroxidation that has been implicated in many processes of tissue injury. The changes in the contents of mevinolin and CoQ10 were analysed by HPLC-MS during solid-substrate (rice, germinated brown rice, white soybean, and seoritae) fermentation with Monascus pilosus KCCM 60084 and Aspergillus terreus KCCM 12225 at 30℃ for 21 days. The significant increase (P<0.05) of mevinolin was observed in A. terreus-fermented solid substrates compared with M. pilosus-fermented solid substrates. The highest yields of 872.2 μg per g dry weight seoritae (black soybean) were obtained after 21 days of fermentation. However, CoQ10 content increased by 1.9 times (72.0 μg/g) in M. pilosus-fermented seoritae after 7 days of fermentation than those fermented with A. terreus. Citrinin was not detected in all samples. Thus, Aspergillus terreus KCCM 12225 was suitable for mevinolin, Monascus pilosus KCCM 60084 was suitable for CoQ10 production, respectively. Seoritae was found to be the best substrate among rice, germinated brown rice, and white soybean for both mevinolin and CoQ10 production. The results indicate that Monascus pilosus KCCM 60084- or Aspergillus terreus KCCM 12225-fermented soybean has potential as multi-funtional health food supplement.