http://repository.sungshin.ac.kr/handle/2025.oak/167 2026-04-03T13:08:15Z http://repository.sungshin.ac.kr/handle/2025.oak/8861 Title: Polysaccharide precipitation enhances the preventive effects of ultrasonicated Undaria pinnatifida (Laminariales, Phaeophyta) sporophyll extract against acute skin damage and oxidative stress Author(s): 송경모; 백소정; 김민정; 엄수진; 이재훈; 이새롬; 강민철; 이남혁; 정성근 Abstract: Although Undaria pinnatifida sporophyll is rich in beneficial components such as polysaccharides, proteins, and phytochemicals,its inaccessible structure limits its usability. To overcome this limitation, we ultrasonically extracted U. pinnatifidasporophyll extract (UPE) under optimized conditions (20 kHz, 1,080 W, 30¡ÆC) for 8 h and used gradient ethanolprecipitation to isolate the UPE fractions (UEF). The yield, composition, and molecular weight of the UEFs changed asfractionation progressed, and the galactose and fucose content increased. Interestingly, the sulfate polysaccharide contentswere not related to the fractionation process: UEF3 showed the highest content among the fractions (33.89 ¡¾ 0.05%). UEFs had no cytotoxic effects on HaCaT, RAW 264.7, or BEAS-2B cells, and UEF3 prevented ultraviolet B (UVB)-inducedcyclooxygenase-2 (COX-2) expression by regulating the ERK kinase pathway. The oral administration of UEF3 preventedepidermal thickening and COX-2 overexpression in UVB-exposed mice. UEF3 also alleviated oxidative stress by reducingreactive oxygen species levels via the activation of the HO-1 / NRF2 signaling pathway. This indicates that polysaccharideprecipitation fractionation enhances efficacy by isolating the functional components. In conclusion, fractional processingusing ultrasonic-assisted extr 2025-06-14T15:00:00Z http://repository.sungshin.ac.kr/handle/2025.oak/8830 Title: Creation of the Ulvan Lyase Enzyme Complex for the Production of Ulvan-Derived Oligosaccharides Author(s): 현정은; 조예원; 송수민; 정지현; 이지현; 한성옥 Abstract: Ulvan, a primary constituent of green algae biomass, has the potential to be used for various biomedical and biotechnology applications. Its biological activity is significantly influenced by its molecular weight. However, the high molecular weight of ulvan poses challenges due to its high viscosity and poor solubility, necessitating an efficient depolymerization process. The ulvan oligosaccharide, produced by decomposing ulvan, not only retains the diverse biological activities of ulvan but also overcomes the issues of low solubility and diminished bioavailability. In this study, ulvan lyase and unsaturated beta-glucuronyl hydrolase were engineered by attaching dockerin to the C-terminus of the enzymes. The resultant ulvan lyase enzyme complex was then reacted with ulvan extracted from biomass to evaluate its depolymerization efficacy. The low-molecular-weight ulvan lysate generated by this enzyme complex showed enhanced prebiotic activity compared to lysate from a single-subunit enzyme. These findings suggest that further decomposition of ulvans via ulvan lyase complex can increase the practical use of ulvan-derived oligosaccharides. Hence, employing electrostatic dockerin-cohesin interactions in seaweed polysaccharide depolymerase complex proves to be effective, offering significant promise for advancing biot 2025-07-01T15:00:00Z http://repository.sungshin.ac.kr/handle/2025.oak/8823 Title: Edible insects as sustainable food sources: extraction techniques, nutritional profiles, and volatile characteristics Author(s): 장혜원; 민유림; 남진경 Abstract: Edible insects have gained considerable attention as sustainable and nutrient-rich protein sources in the food industry. This review summarizes key advancements in edible insect protein and oil extraction techniques. Efficient protein recovery techniques are discussed. In addition, strategies such as defatting (which enhances extraction efficiency) are also discussed. The nutritional significance of edible insect-derived peptides, amino acids, and fatty acids is also reviewed, with emphasis on their potential as food ingredients. Analytical techniques for characterizing edible insect volatile compounds are described, along with the key identified volatiles. Furthermore, fermentation has been explored as a strategy to enhance the consumer acceptance of edible insects. These insights support the development of innovative and sustainable food products that utilize edible insect resources. 2025-04-24T15:00:00Z http://repository.sungshin.ac.kr/handle/2025.oak/8816 Title: Optimization of enzymatic extraction of astaxanthin from Xanthophyllomyces dendrorhous using a recombinant cellulase Author(s): 현정은; 이유경; 한성옥; 고영진 2025-06-19T15:00:00Z